RotaC2.0automated genotyping tool for
Group A rotaviruses. Read More
Group A rotaviruses are the most common cause of severe diarrhea in infants and
children worldwide and continue to have a major global impact on childhood morbidity
and mortality. In recent years, considerable research efforts have been devoted to the
development of two new live, orally administered vaccines. Although both vaccines have
proven to confer a good protection against severe rotavirus gastroenteritis, these vaccines
will have to be screened and may have to be updated regularly to reflect temporal and spatial
genotype fluctuations. In this matter, the genetic characterization of circulating and new
emerging rotavirus strains will need to be compulsory and accurate. An extended classification
system for rotaviruses in which all the 11 genomic RNA segments are used, has been proposed
recently. The use of this classification system will help to elucidate the role of gene reassortments
in the generation of genetic diversity, host range restriction, co-segregation of certain gene
segments, and in adaptation to a new host species.
Here we present a web-based tool that can be used for fast rotavirus genotype differentiation of all 11 group A rotavirus gene segments according to the new guidelines proposed by the Rotavirus Classification Working Group (RCWG).
With the increasing sequencing efforts that are being conducted around the world to unravel complete rotavirus genomes of human and animal origin, this tool will be of great help to analyze and correctly classify the large amount of new data.